In Vitro Model of Essential Fatty Acid Deficiency

Essential fatty acid deficiency and adrenal cortical function in vitro.

Adrenocortical cells were prepared from rats maintained on essential fatty acid-deficient diets and control litter mates. Cells from control rats had high concentrations of essential fatty acids in the cholesteryl ester fraction of which approximately 22% was arachidonate. In contrast, cells from EFA-deficient rats had only 2.5% arachidonate in the cholesteryl esters, even though the total este...

Functional Characterization of an in Vitro Model of Essential Fatty Acid Deficiency in Intestinal Epithelial Cells

Diagnosing essential fatty acid deficiency.

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Fat Absorption in Essential Fatty Acid Deficiency*

The various gross symptoms of essential fatty acid deficiency in the rat are well recognized, but little is known about the metabolic features of this disease. The purpose of this investigation was to study the influence of fat deficiency on the rate of absorption and transport of fat through the intestinal mucosa. The scheme of fat labeling by the use of spectroscopically active methyl esters ...

An in vitro model for essential fatty acid deficiency: HepG2 cells permanently maintained in lipid-free medium.

A stable essential fatty acid-deficient cell type, known as HepG2-EFD, was derived from the lipoprotein-producing human hepatoma cell line HepG2. These cells are particularly useful for quantitative studies involving essential fatty acids (n-6 and n-3 fatty acids) in secreted lipoproteins. Radiolabeled essential fatty acids can be delivered to these cells without altering the specific activity ...